- DMF: 20 mg/ml
- DMSO: 5 mg/ml
- Ethanol: 20 mg/ml
Cryopreserved pooled primary human hepatocyte (HHep) incubations
Hepatocyte incubation and metabolite identification experiments were performed
according to a published procedure33, 34 with slight modifications. Briefly, 5 μmol/L ADB-BUTINACA (obtained from the National Forensic Centre, Sweden) and
(S)-ADB-4en-PINACA (synthesized at Linköping University, see above) were incubated with HHeps (1 × 105 cells in 100 μl) in a total volume of 100 μl.
The synthesis and in vitro pharmacological evaluation of ADB-BUTINACA and ADB-4en-PINACA has been reported recently.20, 29-31
analysis by ultra-high pressure liquid chromatography coupled to high resolution accurate mass spectrometry (UHPLC-HRAM-MS, ADB-BUTINACA, and ADB-4en-PINACA)
Methyl 2-[[1-(4-fluorobutyl)indazole-3-carbonyl]amino]-3,3- dimethyl-butanoate, methyl 2-(1-(4-fluorobutyl)-1H-indazole-3-carboxamido)-3,3-dimethylbutanoate
|Synthetic cannabinoid receptor agonists|
This substance is included in Schedule II of the 1971 Convention on Psychotropic Substances.